The authors discussed relationships among healers' properties and effects in a healing test. They used cucumber pieces (Cucumis sativus 'white spine type') as a bio-sensor in the healing test and measured the bio-sensor responses using gas and fluorescence measurements (4 wavelengths). After using principal component analysis to remove the measurement disturbance due to the difference of healing ways, the authors found there was a negative correlation between gas J values and healers' ages. This suggested there was a relationship between healers' healing ability and their ages (one of the biological factors). However, it was considered that other measurement disturbance factors, not only the difference of healing ways, should be removed.