This paper reports on the fabrication and demonstration of a novel autonomous centrifugal microfluidic dispenser that microchannels are patterned on the double side of the microchip. The dispenser executes metering of dispensing volume and injection into each target chamber. In a capillary dispenser that was previously reported is unstable, because liquids in the chambers during metering are kept by capillary force. However, the present double side patterning dispenser does not rely on capillary forces, owing to siphon valves, which three-dimensionally cross chambers and microchannels. In the experiments, it was confirmed that the double side patterning dispenser has a high accuracy of dispensing volume. Moreover, higher stability in repeatedly dispensing test was confirmed, in comparison with the capillary dispenser the. Therefore, it is expected to realize high stability and compact analytical systems, which are suitable for POCT, by integrating the dispenser on analytical devices.