In this study, we examined the spatial and temporal frequency tuning of motion-in-depth aftereffect. We changed the spatial frequency of test stimuli and temporal frequency of adapting stimuli to investigate the influence on the motion aftereffect duration. It was found that, in spite of the change of spatial frequency of test stimuli, the maximum motion aftereffect duration was measured when the mean temporal frequency of adapting stimuli was set to 3.83 Hz. On the other hand, in spite of the change of temporal frequency of adapting stimuli, the maximum motion aftereffect duration was measured when the mean spatial frequency of test stimuli was set to 0.40 cycles/degree. We also changed the moving direction of adapting stimuli in approaching and receding directions to investigate the moving direction influence on the motion aftereffect duration. It was noted that compared to the approaching motion, longer motion aftereffect duration was measured for the receding motion.