Two cases of solitary colonic tuberculosis were diagnosed by positive culture of tubercle bacilli from the specimen, biopsied through colnof iberscopy. Case 1 is a 52 year-old female. The lesions were present at the proximal part of the ascending colon and the coecum. Roentgenographic and endoscopic findings suggested the presence of colon tuberculosis. Histology of biopsied specimen showed Langhans giant cells, but there was not caesation necrosis. Case 2 is a 27 year-old female. The lesions located at the both of transverse and ascending colon. Roentgeno-graphic and endoscopic findings were also suggestive of tuberculosis. However, histology did not show the typical views of the tuberculosis. Chest X-ray was within normal findings in both cases. These 2 cases were diagnosed finaly by cultured tubercle bacilli from biopsied specimen and were responsive to anti tuberculous chemotherapy. Following procedures should be necessary for the diagnosis of colon tuberculosis without operation ; the presence of tuberculous granuloma, positive acid-fast bacillus stain or positive culture of tubercle bacilli using biopsied specimen. In general, positive acid-fast bacillus stain for tubercle bacilli is rare, and biopsy is not always successful in picking up the tuberculous granuloma. Therefore, it seems to be very important for the diagnosis of colon tuberculosis to prove the presence of the tubercle bacilli in the biopsied specimen which were taken by colonofiberscope, using culture system for tubercle bacilli.