Using a berberine-aniline blue dyeing procedure, the development of Casparian strips from the roots of four evergreen broadleaved tree species (Osmanthus fragrans, Cinnamomum camphora, Machilus thunbergii and Enonymus japonicus) was observed. This dyeing procedure was effective for observing the Casparian strips from the roots of the four species. ‘The Casparian strips of the exodermis were formed near the position where one of the endodermis was formed. The positions where these Casparian strips were formed did not relate to the salt tolerance of these species.